degenerate PCR

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Describing a polymerase chain reaction (PCR) in which the primers are not specific but are deliberately mixed (degenerate primers—see degeneracy) to amplify a sequence that has been deduced from the peptide sequence (so the exact codon usage is unknown) or to isolate homologous genes. A similar approach, using deliberately degenerate sequences, is also used in screening genomic libraries.

Subjects: Medicine and Health.

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