2′,3′-dideoxynucleoside triphosphates

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Analogs of normal 2′-deoxyribonucleoside triphosphates used in a modified “minus” technique for base sequencing of DNA molecules. Because these analogs have no oxygen at the 3′ position in the sugar, they act as specific chain-terminators (q.v.) for primed synthesis techniques (see DNA sequencing techniques). Nucleotides in which arabinose is substituted for deoxyribose also exhibit this chain-terminating effect.

Subjects: Genetics and Genomics.

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