Overview

differential display PCR


'differential display PCR' can also refer to...

differential display PCR

differential display PCR

Differential Display Pcr: a New Age in Nutrition Investigation

Primer design for a prokaryotic differential display RT-PCR

Rational primer design greatly improves differential display-PCR (DD-PCR)

A computer-driven approach to PCR-based differential screening, alternative to differential display.

Interaction and effect of annealing temperature on primers used in differential display RT-PCR

Efficient reamplification of differential display products by transient ligation and thermal asymmetric PCR

Identification of stress-responsive genes in Caenorhabditis elegans using RT-PCR differential display

Identification of mood stabilizer-regulated genes by differential-display PCR

Isolation of genes negatively or positively co-expressed with human recombination activating gene 1 (RAG1) by differential display PCR (DD RT-PCR)

Identification and partial characterization of differentially expressed mRNAs in normal human endometria and endometrial carcinomas by differential display RT–PCR

Direct identification of differentially expressed genes by cycle sequencing and cycle labelling using the differential display PCR primers

Identification of quorum sensing-related regulons in Vibrio vulnificus by two-dimensional gel electrophoresis and differentially displayed reverse transcriptase PCR

Differences in gene expression of the ciliate Paramecium caudatum caused by endonuclear symbiosis with Holospora obtusa, revealed using differential display reverse transcribed PCR

Identification and expression profiling of low oxygen regulated genes from Citrus flavedo tissues using RT-PCR differential display

 

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A method, based on the polymerase chain reaction, used to identify genes differentially expressed in various tissues or in normal vs pathological states. The messenger RNA from the two samples is reverse transcribed, amplified with rather nonspecific primers, and run on a high-resolution gel. Bands unique to single samples are considered to be differentially expressed and can be used to clone the full-length cDNA. Superseded by gene chip technologies. See also differential screening.

Subjects: Chemistry — Medicine and Health.


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