Overview

DNA filter assay


'DNA filter assay' can also refer to...

 

More Like This

Show all results sharing this subject:

  • Biological Sciences

GO

Show Summary Details

Quick Reference

A method used to determine the presence of recombinant DNA in cloned cells. This is necessary as a culture will contain many cells that have not been transformed. After the culture has been plated on Petri dishes a copy of the cell pattern is made using a special filter and the cells are then lysed. DNA from the cells binds to the filter as it is heated in a pattern equivalent to that of the cells on the Petri dish. The presence of recombinant DNA is revealed by the use of a gene probe and autoradiography.

Subjects: Biological Sciences.


Reference entries

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.