Eadie-Hofstee plot

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A graphical method for analysing enzyme kinetics. The velocity of reaction (V) is plotted on the ordinate, V/S on the abscissa, S being the initial substrate concentration. The intercept on the ordinate is Vmax, the slope is −Km. A better method than using the Lineweaver–Burke plot.

Subjects: Chemistry — Medicine and Health.

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