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electrochemiluminescence


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(ECL)

Light emission stimulated by an electrochemical reaction (electrogenerated chemiluminescence). In screening assays it is convenient to arrange that the electrochemical reaction will only occur if the components are physically adjacent, e.g. when labelled ligand binds to an immobilized receptor: Following electrical stimulation the light emission is then a measure of binding. A common label is tris(bipyridine)ruthenium(II), NHS ester.

Subjects: Medicine and Health.


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