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enhancer trap


'enhancer trap' can also refer to...

enhancer trap

enhancer trap

enhancer trap vector

enhancer trap vector

Enhanced gene trapping in mouse embryonic stem cells

C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin

Modification of the Collection Solvent to Enhance Liquid Trapping Efficiencies After Supercritical Fluid Extraction

GAL4 GFP enhancer trap lines for analysis of stomatal guard cell development and gene expression

A Thermonuclease of Neisseria gonorrhoeae Enhances Bacterial Escape From Killing by Neutrophil Extracellular Traps

GAL4-GFP enhancer trap lines for genetic manipulation of lateral root development in Arabidopsis thaliana

Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish

Gene and Enhancer Trap Transposable Elements Reveal Oxygen Deprivation‐regulated Genes and their Complex Patterns of Expression in Arabidopsis

Enhanced Attraction of Plutella xylostella (Lepidoptera: Plutellidae) to Pheromone-Baited Traps With the Addition of Green Leaf Volatiles

Enhanced sequence coverage of proteins in human cerebrospinal fluid using multiple enzymatic digestion and linear ion trap LC-MS/MS

Enhanced Selectivity in the Determination of Δ9-Tetrahydrocannabinol and Two Major Metabolites in Serum Using Ion-Trap GC-MS-MS

Enhanced capture of extramembranous IgM and IgG on B cells in the NOD mouse—implications for immune complex trapping

P694Repeated social defeat enhances neutrophil extracellular traps formation in vivo and in vitro: Potential implication in the exaggerated atherosclerosis in chronic social stress-exposed apoE−/− mice

 

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A technique used in Drosophila to demonstrate the occurrence of enhancers that switch on genes in specific groups of cells during certain developmental periods. A reporter gene (q.v.) is used, which has a promoter that requires the assistance of an enhancer to be activated. The reporter gene, together with its “weak” promotor, is spliced into a transposable element (q.v.). In Drosophila, P elements (q.v.) are used, and these insert themselves at various chromosomal sites. When insertion occurs near an enhancer that normally activates genes in specific areas of a developing embryo, the reporter gene intercepts the signal and reports the position of the cells by its activity. In cases where the reporter is lacZ, blue pigment appears in specific cells; for example, in localized regions of the developing central nervous system. Once insertions that generate interesting staining patterns are identified, stable strains of flies carrying the insertions can be produced. Such lines are called transposants. Cytological mapping has shown that insertions occur throughout the genome. Since the enhancers are generally positioned within a few hundred base pairs of the start site of their target genes, these can be subsequently cloned and sequenced. See Chronology, 1987, O'Kane and Gehring.

Subjects: Genetics and Genomics.


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