A technique used to show T cells in leucocyte mixture. Sheep erythrocytes (‘E’) will bind to form a ‘rosette’ around T cells whereas B cells, which have Fc receptors, require the erythrocytes to be antibody (A) treated and will then form EA rosettes. Antibody-coated erythrocytes with bound complement (C) will form EAC rosettes around cells with C3b or C3bi receptors (CR1 or CR3). EAC rosettes form more easily than E or EA rosettes.
Subjects: Medicine and Health.