ethidium bromide

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A compound used to separate covalent DNA circles from linear duplexes by density gradient centrifugation. Because more ethidium bromide is bound to a linear molecule than to a covalent circle, the linear molecules have a higher density at saturating concentrations of the chemical and can be separated by differential centrifugation. It is also used to locate DNA fragments in electrophoretic gels because of its fluorescence under ultraviolet light.

Subjects: Medicine and Health — Genetics and Genomics.

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