A blotting method to assess protein–protein interactions. Proteins are transferred from a gel to a membrane (often nitrocellulose), allowed to renature and are then probed with the putative interacting protein. Binding is usually detected with antibody to the binding protein.
http://www.piercenet.com/Proteomics/browse.cfm?fldID=BDAE338C-43BF-4FBA-B7D3-56C477BAEB9B Methodological details on blotting techniques.
Subjects: Medicine and Health.