far Western blot

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A blotting method to assess protein–protein interactions. Proteins are transferred from a gel to a membrane (often nitrocellulose), allowed to renature and are then probed with the putative interacting protein. Binding is usually detected with antibody to the binding protein.

http://www.piercenet.com/Proteomics/browse.cfm?fldID=BDAE338C-43BF-4FBA-B7D3-56C477BAEB9B Methodological details on blotting techniques.

Subjects: Medicine and Health.

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