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Ficoll


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A high molecular weight branched polysaccharide used to produce solutions of high viscosity and low osmotic pressures for the density-gradient separation of leucocytes from whole blood. Ficoll-Paque is premixed Ficoll and Hypaque (diatrizoate) with a density greater than that of all blood cells other than lymphocytes (1.077 g/cm3). This allows a one-step centrifugation process for isolating lymphocytes which remain at the plasma–Ficoll-Paque boundary.

Subjects: Chemistry — Medicine and Health.


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