A method for the isolation of nutritional mutants in fungal genetics. Mutagenized spores are placed upon a minimal medium. Normal spores germinate and send out an extensive mycelial network. These colonies are then filtered off, and the remaining germinated spores that show poor mycelial development are grown upon a supplemented medium, where each produces enough mycelia to allow further propagation and study.
Subjects: Chemistry — Genetics and Genomics.