flying spot cytometer

Quick Reference

An instrument used in cytometric DNA measurements. The heterogenous distribution of Feulgen stain within a nucleus leads to distributional errors when a single measurement is made of light transmission to estimate the amount of dye bound in the nucleus. Flying spot cytometers scan a defined microscopic area while making thousands of measurements with a minute measuring spot. The sum of these point absorbance measurements as determined by a built-in computer is proportional to the true absorbance of the specimen. See Feulgen procedure, microspectrophotometer.

Subjects: Genetics and Genomics.

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