A method for the fixation of histological specimens, involving a minimum of chemical and physical change. Specimens are immersed in isopentane cooled to –190°C in liquid air. This fixes the tissue instantly, without the formation of large ice crystals (which would cause structural changes). The tissue is then dehydrated in a vacuum for about 72 hours at –32.5°C.
Subjects: Medicine and Health — Science and Mathematics.