freeze drying

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A method for the fixation of histological specimens, involving a minimum of chemical and physical change. Specimens are immersed in isopentane cooled to –190°C in liquid air. This fixes the tissue instantly, without the formation of large ice crystals (which would cause structural changes). The tissue is then dehydrated in a vacuum for about 72 hours at –32.5°C.

Subjects: Medicine and Health — Science and Mathematics.

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