A method formerly used for sterilizing the working ends of endodontic files and reamers by placing them in a container containing glass beads heated to approximately 225°C (437°F) for a defined period of time. Glass bead sterilizers are ineffective against prions, and the effectiveness of the glass bead sterilizer for viral infection control has not been demonstrated unequivocally. The effectiveness of glass bead sterilizers is very difficult to validate. Current cross-infection control and prevention best practice requires that endodontic files are single-use items only and should not be processed for reuse under any circumstances. Many manufacturers of endodontic files specify that the items are for single use only.