A method used to detect cells secreting antibody in vitro. Sheep red cells, treated so that they will bind the antibody and be lysed, are mixed with the cell suspension, immobilized in agarose, and incubated. Antibody-producing cells will lyse adjacent erythrocytes, forming a plaque of haemolysis. The assay method can be adapted to identify other types of cells, e.g. those secreting a protein that will generate an immune complex with added antibody, activate complement and cause haemolysis.
Subjects: Medicine and Health.