Overview

his tag


'his tag' can also refer to...

his tag

His‐tag

His‐tag antibody

His-tag antibody

Controlled specific expression and purification of 6×His-tagged proteins in Pseudomonas

His-tags as Zn(II) binding motifs in a protein-based fluorescent sensor

Rapid purification of an active recombinant His-tagged 2,3-dihydroxybiphenyl 1,2-dioxygenase from Pseudomonas putida OU83

Expression and rapid one-step purification of biologically active His-tagged factor C by Ni2+ affinity column chromatography

Highly Purified Thermo-Stable Oxygen-Evolving Photosystem II Core Complex from the Thermophilic Cyanobacterium Synechococcus elongatus Having His-Tagged CP43

Properties of Chlamydomonas Photosystem II Core Complex with a His-Tag at the C-Terminus of the D2 Protein

A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli

Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coli

Over-expression of recombinant proteins with N-terminal His-tag via subcellular uneven distribution in Escherichia coli

On-column Refolding of an Insoluble His6-tagged Recombinant EC-SOD Overexpressed in Escherichia coli

Expression and characterization of two secreted His6-tagged endo-β-1,4-glucanases from the mollusc Ampullaria crossean in Pichia pastoris

Binding and Functional Properties of the Extrinsic Proteins in Oxygen-Evolving Photosystem II Particle from a Green Alga, Chlamydomonas reinhardtii having His-tagged CP47

Nucleotide sequence of the gene encoding cis-biphenyl dihydrodiol dehydrogenase (bphB) and the expression of an active recombinant His-tagged bphB gene product from a PCB degrading bacterium, Pseudomonas putida OU83

 

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Quick Reference

An epitope tag (histidine tag) based on a short stretch (~6) of histidine residues added to either the N- or C-terminus of a protein, sometimes with an added region susceptible to endopeptidase cleavage to allow stripping of the tag from the recombinant protein. Addition of the tag allows detection with antipolyhistidine antibodies and protein purification on nickel-based affinity columns.

Subjects: Medicine and Health — Chemistry.


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