his tag

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Quick Reference

An epitope tag (histidine tag) based on a short stretch (~6) of histidine residues added to either the N- or C-terminus of a protein, sometimes with an added region susceptible to endopeptidase cleavage to allow stripping of the tag from the recombinant protein. Addition of the tag allows detection with antipolyhistidine antibodies and protein purification on nickel-based affinity columns.

Subjects: Medicine and Health — Chemistry.

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