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HTRF


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An assay method (homogeneous time-resolved fluorescence) in which an absorbing fluorochrome is coupled to one component, an emitting fluorochrome with slow-release characteristics coupled to the other: binding will allow fluorescence energy transfer between the fluorochromes and the light emission, analysed in a time-resolved fluorescence system, is a measure of binding. Because no separation is required the system lends itself to high-throughput screening for inhibitors of binding interactions.

Subjects: Medicine and Health.


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