hybridization competition

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A technique for distinguishing different mRNA molecules, using a variation of the basic filter hybridization (q.v.) technique. A specific DNA sequence trapped on a nitrocellulose filter is exposed to a tritiated RNA known to be complementary to that DNA. An unlabeled RNA of unknown specificity is added and, if complementary to the DNA, it will compete with the labeled molecules for hybridization to the DNA. Any diminution of labeling in the hybrid after equilibrium is reached is attributed to displacement by the unlabeled molecules.

Subjects: Genetics and Genomics.

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