in situ hybridization

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A technique utilized to localize, within intact chromosomes, eukaryotic cells, or bacterial cells, nucleic acid segments complementary to specific labeled probes. To localize specific DNA sequences, specimens are treated so as to denature DNAs and to remove adhering RNAs and proteins. The DNA segments of interest are then detected via hybridization with labeled nucleic acid probes. The distribution of specific RNAs within intact cells or chromosomes can be localized by hybridization of squashed or sectioned specimens with an appropriate RNA or DNA probe. See Chronology, 1969, Gall and Pardue; 1975, Grunstein and Hogness; 1981, Harper and Saunders; 1983, Hafen, Levine, and Gehring; chromosome painting, fluorescence in situ hybridization (FISH).

Subjects: Genetics and Genomics — Medicine and Health.

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