in vitro evolution

'in vitro evolution' can also refer to...

in vitro evolution

in vitro evolution

Frame shuffling: a novel method for in vitro protein evolution

Diversification and Specialization of HIV Protease Function During In Vitro Evolution

In vitro evolution of distinct self-cleaving ribozymes in diverse environments

In vitro evolution of flexizymes that function under the conditions in translation system


Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization

Covalent DNA display as a novel tool for directed evolution of proteins in vitro

In vitro evolution of an antibody fragment population to find high-affinity hapten binders

Changing the recognition specificity of a DNA-methyltransferase by in vitro evolution

Random-priming in vitro recombination: An effective tool for directed evolution

In Vitro Evolution of a Polyhydroxybutyrate Synthase by Intragenic Suppression-Type Mutagenesis

Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution

Evolution of 2-long terminal repeat (2-LTR) episomal HIV-1 DNA in raltegravir-treated patients and in in vitro infected cells

Changes in in vitro susceptibility of influenza A H3N2 viruses to a neuraminidase inhibitor drug during evolution in the human host

Identification of Essential Amino Acid Changes in Paired Domain Evolution Using a Novel Combination of Evolutionary Analysis and In Vitro and In Vivo Studies

An improved method for the in vitro evolution of aptamers and applications in protein detection and purification

Functional humanization of an anti-CD30 Fab fragment for the immunotherapy of Hodgkin's lymphoma using an in vitro evolution approach


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Experiments designed to study the evolution of self-duplicating nucleic acid molecules outside of living cells. A classic example is a study that involved the synthesis of RNA molecules using Qβ replicase and the RNA genome of Qβ phage. Serial transfer experiments were performed in which the intervals of synthesis were adjusted to select the earliest molecules completed. As the experiment progressed, the rate of RNA synthesis increased, while the product became smaller. By the 74th transfer, an RNA molecule had evolved that was only 17% of its original size and constituted the smallest known self-duplicating molecule. While it had a very high affinity for the replicase, it was unable to direct the syntheses of viral particles. Ribozymes (q.v.) have also been shown to undergo in vitro evolution. See Chronology, 1967, Spiegelmann, Mills, and Peterson; 1973, Mills, Kramer, and Spiegelmann; 1995, Wilson and Szostak.

Subjects: Genetics and Genomics.

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