Overview

Klenow fragment


Show Summary Details

Quick Reference

The larger of two fragments obtained by the enzymatic cleavage of the DNA polymerase I of E. coli. The drawing below shows that the Klenow fragment lacks 5′ → 3′ exonuclease activity. Because of this, the Klenow fragment has various uses in genetic engineering techniques and in DNA sequencing methodologies where the 5′ → 3′ exonuclease activity of intact DNA polymerase I is disadvantageous. See Chronology, 1971, Klenow; DNA polymerase.

Subjects: Chemistry — Genetics and Genomics.


Reference entries

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.