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Maxam–Gilbert method


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A DNA sequencing method in which DNA is degraded, in a set of independent, nucleotide-specific reactions, to produce fragments with characteristic sizes that can be resolved on a sequencing gel. Maxam–Gilbert sequencing has advantages, e.g. for oligonucleotides or covalently modified DNA, although dideoxy sequencing is more commonly used.

Subjects: Medicine and Health.


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