Chromosomal whole mounts spread for electron microscopy by a method developed by O. L. Miller. In this method, chromosomes from ruptured nuclei are centrifuged through a solution of 10% formalin in 0.1 mole sucrose and onto a membrane-coated grid. The grid is then treated with an agent that reduces the surface tension as the grid dries. After being stained with phosphotungstic acid, the preparation is ready for viewing under the electron microscope.
Subjects: Genetics and Genomics.