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mitotic shake-off method


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A method for selecting cells undergoing mitosis to allow karyotyping. Many cells round up during mitosis and are less firmly attached to the culture substratum, so that gentle shaking of the culture vessel will detach the mitotic cells. The proportion in mitosis is usually increased by adding a drug that blocks cells at metaphase (e.g. colchicine). The method can also be used to obtain a reasonably well synchronized population.

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