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monolayering of cells


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The tendency of cells grown in culture to form a single layer of cells covering the surface (substratum). Most normal (nontransformed) cells do not form multilayers in culture, partly because contact inhibition of locomotion causes cells to change their direction of movement when they collide with another cell, partly because proliferation is limited by the supply of growth factors that must reach the cells by diffusion through the medium above them (the diffusion boundary layer). Proliferation continues after a confluent monolayer is formed and the limit is a density effect, rather than a contact phenomenon (see density dependent inhibition of growth).

Subjects: Medicine and Health.


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