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multiplex PCR


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A type of polymerase chain reaction (q.v.) that is used to sample various regions of a large gene from one end to the other. For example, to analyze the human dystrophin gene, which occupies over 2 million base pairs on the X chromosome, multiplex PCR might involve simultaneous amplification from nine different sets of primers, all within the same reaction test tube. Each set of primers is chosen to produce a different-sized amplification product from a different region of the dystrophin gene. Normal males will display nine characteristic bands after the amplification products are separated by gel electrophoresis. Males with deletions in the dystrophin gene will be missing one or more of these bands. See muscular dystrophy.

Subjects: Genetics and Genomics.


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