Overview

negative staining


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A staining technique for high-resolution electron microscopy of viruses. A virus suspension is mixed with a phosphotungstic acid solution and poured into an atomizer sprayer. The mixture is then sprayed upon electron microscope grids previously coated with a film of carbon. The phosphotungstic acid enters the contours of the specimen, which is viewed as a light object against a dark background. See Chronology, 1959, Brenner and Horne.

Subjects: Genetics and Genomics.


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