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Ouchterlony assay


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An immunological assay for antigen–antibody reactions in which radial diffusion of soluble antigen and antibody from wells cut in a gel leads to the formation of an antigen–antibody complex, visible usually as a whitish band, where the antibody/antigen ratio is appropriate. The method is tolerant of wide variation in concentrations of antibody and antigen.

Subjects: Medicine and Health.


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