A measure of the extent of multilayering of cells in culture. The actual extent of overlapping, usually of nuclei, is measured on fixed and stained preparations and compared to the predicted level based on cell density, the projected area of the nucleus and the assumption of a Poisson distribution. A value of 1 implies a random distribution but a constraint on overlapping, as with normal fibroblasts that exhibit contact inhibition of locomotion, may produce a value as low as 0.05. The reduction of overlapping may also arise though the relative nonadhesiveness of the dorsal surfaces of cells.
Subjects: Medicine and Health.