Overview

photoactivated crosslinking


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A technique for crosslinking a nucleic acid (e.g., a tRNA chain) to a polypeptide chain with which it is functionally associated (e.g., its cognate synthetase) by irradiating the synthetase-tRNA complex with ultraviolet light. The technique is used to locate the points of intimate contact between the two molecules. See RNase protection.

Subjects: Genetics and Genomics.


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