A technique used to detect DNA segments in one sample (the tester pool) that are not in another (the driver pool). DNAs from each pool are fragmented by digestion with restriction endonucleases (q.v.), and the fragments are amplified by the polymerase chain reaction (q.v.). The amplicons from one pool are then allowed to hybridize with those from the other, and the hybrid molecules are removed. This subtractive hybridization leads to the enrichment of fragments that are unique to the tester pool. Such a technique could be used to isolate cDNAs from genes specifically activated by certain hormones.
Subjects: Genetics and Genomics.