An enzyme made up of 124 amino acids, first isolated from bovine pancreas. Ribonuclease A hydrolyzes pyrimidines at the 3′ phosphate group and cleaves the 5′ phosphate linkage to the adjacent nucleotide. The end products of digestion are pyrimidine 3′ phosphates and oligonucleotides with pyrimidine 3′ phosphate termini. Ribonuclease A was the first protein to be subjected to reversible chemical modifications that demonstrated that the linear sequence of amino acids determined the unique three-dimensional structure of the protein. See Chronology, 1961, Anfinsen et al.
Subjects: Genetics and Genomics.