Overview

R-loop mapping


Show Summary Details

Quick Reference

A technique for visualizing under the electron microscope the complementary regions shared by a specific eukaryotic RNA and a segment of one strand of a DNA duplex. The RNA-DNA hybrid segment displaces one of the DNA strands, causing it to form a loop; hence the name of the technique. Double-stranded regions appear thicker than single-stranded regions in electron micrographs. Introns cannot hybridize with mature mRNA (from which introns have been removed); thus, one intron results in two R loops, two introns yield three R loops, etc.

See Chronology, 1977, Chow and Berget.

R-loop mapping

Subjects: Genetics and Genomics.


Reference entries

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.