Overview

site-directed mutagenesis


'site-directed mutagenesis' can also refer to...

site-directed mutagenesis

site-directed mutagenesis

site‐directed mutagenesis

Cloning, Characterization and Site-Directed Mutagenesis of Canine Renin

Functional Expression and Site-Directed Mutagenesis of Photoactive Yellow Protein

Computational site-directed mutagenesis of haloalkane dehalogenase in position 172.

Study of B72.3 combining sites by molecular modeling and site-directed mutagenesis

Molecular modelling and site-directed mutagenesis of the active site of endothelin-converting enzyme.

Site-directed mutagenesis of active site residues in a class I endochitinase from chestnut seeds

An efficient one-step site-directed and site-saturation mutagenesis protocol

Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima)

Site-directed mutagenesis of the cysteine residues in the Pichia stipitis xylose reductase

Primary Structure, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from Bacillus stearothermophilus

Molecular Cloning, Enhancement of Expression Efficiency and Site-Directed Mutagenesis of Rat Epidermal Cystatin A

Alternative method for site-directed mutagenesis of complex polyketide synthase in Streptomyces albus JA3453

Homology modelling and site-directed mutagenesis studies of the epoxide hydrolase from Phanerochaete chrysosporium

Molecular Mechanisms of Improvement of Hydrolytic Antibody 6D9 by Site-Directed Mutagenesis

Molecular Cloning, Expression, and Site-Directed Mutagenesis of Inorganic Pyrophosphatase from Thermus thermophilus HB8

Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis

 

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Quick Reference

A technique used in genetic engineering for introducing particular changes to the base sequence of a gene at a specific site. It allows precise and specific mutations to be made and is done, for example, to modify the amino-acid sequence of the protein expressed by the gene to investigate how such a change affects the protein's structure and function. First, the gene of interest is cloned and made available as single-stranded DNA – a widely used vector for this purpose is the bacteriophage M13. Then an artificial oligonucleotide, containing perhaps 20–30 nucleotides, is constructed containing the desired change in base sequence. This is allowed to hybridize with the complementary (apart from the mutation site) single-stranded DNA and is then extended at either end by the enzyme DNA polymerase using the single-stranded DNA as template. The two strands, each with their vector, are then separated and cloned, and clones containing the mutant gene are selected.

Subjects: Biological Sciences.


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