Overview

site-directed mutagenesis


'site-directed mutagenesis' can also refer to...

site-directed mutagenesis

site‐directed mutagenesis

site-directed mutagenesis

Computational site-directed mutagenesis of haloalkane dehalogenase in position 172.

Cloning, Characterization and Site-Directed Mutagenesis of Canine Renin

Functional Expression and Site-Directed Mutagenesis of Photoactive Yellow Protein

Study of B72.3 combining sites by molecular modeling and site-directed mutagenesis

Molecular modelling and site-directed mutagenesis of the active site of endothelin-converting enzyme.

Site-directed mutagenesis of active site residues in a class I endochitinase from chestnut seeds

An efficient one-step site-directed and site-saturation mutagenesis protocol

Site-directed mutagenesis and CBM engineering of Cel5A (Thermotoga maritima)

Site-directed mutagenesis of the cysteine residues in the Pichia stipitis xylose reductase

Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis

Analysis of the psychrotolerant property of hormone-sensitive lipase through site-directed mutagenesis

Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization and site-directed mutagenesis

Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis

Site-directed mutagenesis in hemoglobin: attempts to control the oxygen affinity with cooperativity preserved.

Design and characterization of novel trypsin-resistant firefly luciferases by site-directed mutagenesis

High Efficiency of Site-Directed Mutagenesis Mediated by a Single PCR Product

 

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Quick Reference

A technique used in genetic engineering for introducing particular changes to the base sequence of a gene at a specific site. It allows precise and specific mutations to be made and is done, for example, to modify the amino-acid sequence of the protein expressed by the gene to investigate how such a change affects the protein's structure and function. First, the gene of interest is cloned and made available as single-stranded DNA – a widely used vector for this purpose is the bacteriophage M13. Then an artificial oligonucleotide, containing perhaps 20–30 nucleotides, is constructed containing the desired change in base sequence. This is allowed to hybridize with the complementary (apart from the mutation site) single-stranded DNA and is then extended at either end by the enzyme DNA polymerase using the single-stranded DNA as template. The two strands, each with their vector, are then separated and cloned, and clones containing the mutant gene are selected.

Subjects: Biological Sciences.


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