site-specific mutagenesis

'site-specific mutagenesis' can also refer to...

site-specific mutagenesis

Changes in the specificity of antibodies by site-specific mutagenesis followed by random mutagenesis

Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis

Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis

Site-specific mutagenesis in a homogeneous polyglutamine tract: application to spinocerebellar ataxin-3

In vitro scanning saturation mutagenesis of all the specificity determining residues in an antibody binding site

Characterization of the S1 Subsite Specificity of Aspergillopepsin I by Site-Directed Mutagenesis

Site‐specific mutagenesis by triple helix‐forming oligonucleotides containing a reactive nucleoside analog

Improved efficiency of site-specific copper(II) ion-catalysed protein cleavage effected by mutagenesis of cleavage site

Construction of a new leucine dehydrogenase with preferred specificity for NADP+ by site-directed mutagenesis of the strictly NAD+-specific enzyme.

Low target site specificity of an IS6100-based mini-transposon, Tn1792, developed for transposon mutagenesis of antibiotic-producing Streptomyces

Engineering the Substrate Specificity of Porcine Kidney d-Amino Acid Oxidase by Mutagenesis of the “Active-Site Lid”

Sequence Motif-Specific Assignment of Two [2Fe-2S] Clusters in Rat Xanthine Oxidoreductase Studied by Site-Directed Mutagenesis

Molecular modelling and site-directed mutagenesis of human GALR1 galanin receptor defines determinants of receptor subtype specificity

Structural interpretation of site-directed mutagenesis and specificity of the catalytic subunit of protein kinase CK2 using comparative modelling

Redesign of the coenzyme specificity in l-Lactate dehydrogenase from Bacillus stearothermophilus using site-directed mutagenesis and media engineering

Engineering of α1-antitrypsin variants with improved specificity for the proprotein convertase furin using site-directed random mutagenesis

The role of adduct site-specific mutagenesis in understanding how carcinogen-DNA adducts cause mutations: perspective, prospects and problems

Site-specific Mtm1 mutagenesis by an AAV-Cre vector reveals that myotubularin is essential in adult muscle


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An experimental approach to determining which residues in a protein are important for function. The gene is cloned, specific nucleotide pairs changed to alter a particular codon, and the modified gene is reintroduced into the cell in an expression vector. An alanine scan is a systematic exchange, using this methodology, of every amino acid in turn for alanine, a procedure that is unlikely to make major changes to secondary structure but removes side groups.

Subjects: Medicine and Health.

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