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5.8S rRNA


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5.8S rRNA

5.8S rRNA

Role of the 5.8S rRNA in ribosome translocation

The exosome subunit Rrp43p is required for the efficient maturation of 5.8S, 18S and 25S rRNA

MPP6 is an exosome-associated RNA-binding protein involved in 5.8S rRNA maturation

Secondary structure models of the nuclear internal transcribed spacer regions and 5.8S rRNA in Calciodinelloideae (Peridiniaceae) and other dinoflagellates

The internal transcribed spacers and 5.8S rRNA gene show extensive diversity among isolates of the Cryptococcus neoformans species complex

A new PCR primer for the identification of Paracoccidioides brasiliensis based on rRNA sequences coding the internal transcribed spacers (ITS) and 5·8S regions

Identification of yeast species from orange fruit and juice by RFLP and sequence analysis of the 5.8S rRNA gene and the two internal transcribed spacers

Three oligotrophic bacterial strains were cultured from the ground water of the deep-well monitoring site S15 of the Siberian radioactive waste depository Tomsk-7, Russia. They were affiliated with Actinobacteria from the genus Microbacterium. The almost fully sequenced 16S rRNA genes of two of the isolates, S15-M2 and S15-M5, were identical to those of cultured representatives of the species Microbacterium oxydans. The third isolate, S15-M4, shared 99.8% of 16S rRNA gene identity with them. The latter isolate possessed a distinct cell morphology as well as carbon source utilization pattern from the M. oxydans strains S15-M2 and S15-M5. The three isolates tolerated equal amounts of uranium, lead, copper, silver and chromium but they differed in their tolerance of cadmium and nickel. The cells of all three strains accumulated high amounts of uranium, i.e. up to 240 mg U (g dry biomass)−1 in the case of M. oxydans S15-M2. X-ray absorption spectroscopy (XAS) analysis showed that this strain precipitated U(VI) at pH 4.5 as a meta-autunite-like phase. At pH 2, the uranium formed complexes with organically bound phosphate groups on the cell surface. The results of the XAS studies were consistent with those obtained by transmission electron microscopy (TEM) and energy dispersive X-ray analysis (EDX)

 

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A component of the large ribosomal RNA molecule that is transcribed in the nucleolus. 5.8S rRNA is the structural equivalent of the 5′-terminal 160 nucleotides of prokaryotic 23S rRNAs. Thus, in eukaryotes, the 5.8S and 28S coding sequences are separated by an internal transcribed spacer that is absent from the rDNA unit that is transcribed into the RNA of the large subunit of prokaryotic ribosomes. The 5.8S and 28S molecules are eventually separated by posttranscriptional excision of the spacer. However, these molecules remain associated by intermolecular base pairing interactions as the large subunit of the ribosome matures. See Miller trees, ribosomal RNA genes, ribosome.

Subjects: Genetics and Genomics.


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