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The condition with regard to temperature, ionic strength, and the presence of certain organic solvents such as formamide (q.v.), under which nucleic acid hybridizations are carried out. With conditions of high stringency, pairing will occur only between nucleic acid fragments that have a high frequency of complementary base sequences. Conditions of weaker stringency must be used if the nucleic acids come from organisms that are genetically diverse. Thus, if one were trying to isolate an alcohol dehydrogenase gene from a silkworm genomic library using a cloned gene from Drosophila melanogaster as a probe, less stringent conditions would be used than if the library came from D. virilis.

Subjects: Genetics and Genomics — Chemistry.


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