transposon tagging

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A technique used to isolate mutations induced by the insertion of a transposable element into genes of interest. The mutations are identified by the phenotypic changes that result from their inactivation. The chromosomal locations of the mutations can then be identified by hybridization of the inserted sequences with polynucleotide probes complementary to the transposon. P elements (q.v.) have been used to tag Drosophila genes, and Activator and other maize transposons have served to tag genes from maize and heterologous species. See Chronology, 1994, Whitman et al.; Activator-Dissociation system, tobacco mosaic virus.

Subjects: Genetics and Genomics.

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