Overview

X-Gal


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A compound that is hydrolysed by β-galactosidase to form an intense blue precipitate. X-Gal in conjunction with the inducer IPTG is used to detect recombinants (white) from nonrecombinants (blue) in blue/white colony screening and to detect β-galactosidase reporter gene activity in transfected cells.

Subjects: Chemistry.


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