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Boyden chamber


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A simple design of two-compartment chamber used to test for chemotaxis, especially of leucocytes. The upper compartment, in which cells are placed, is separated from the lower chamber containing the chemotactic factor by a Millipore-type filter (3–8 μm pore size, ~150 μm thick) across which a chemotactic gradient develops. The distance cells have moved into the filter is measured after an incubation period sufficiently short to avoid the gradient decaying. Interpretation is complicated by the fact that most chemotactic factors are also chemokinetic. See also checkerboard assay.

Subjects: Medicine and Health.


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