A method for creating immortalized human antibody-producing cells in vitro without forming a hybridoma (q.v.). Normal B lymphocytes from an immunized donor are mixed with other cells infected with the Epstein-Barr virus (q.v.). The virus enters the B lymphocytes. The cells originally infected with the virus are experimentally destroyed, and the virally transformed cells producing the antibody of interest are isolated. In cell-driven viral transformation, about 1 in 50 B lymphocytes is transformed, whereas with the cell hybridization technique only about 1 human cell in 10 million is transformed.
Subjects: Genetics and Genomics.