colony hybridization

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An in situ hybridization (q.v.) technique used to identify bacteria carrying chimeric vectors whose inserted DNA is homologous with the sequence in question. Colony hybridization is accomplished by transferring bacteria from a petri plate to a nitrocellulose filter. The colonies on the filter are then lysed, and the liberated DNA is fixed to the filter by raising the temperature to 80°C. After hybridization with a labeled probe, the position of the colonies containing the sequence under study is determined by autoradiography (q.v.). See Chronology, 1975, Grunstein and Hogness.

Subjects: Chemistry — Genetics and Genomics.

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