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plasmid cloning vector


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A plasmid used in recombinant DNA experiments as an acceptor of foreign DNA. Plasmid cloning vectors are generally small and replicate in a relaxed fashion. They are marked with antibiotic resistance genes and contain recognition sites for restriction endonucleases in regions of the plasmid that are not essential for its replication. One widely used plasmid cloning vector is pBR322 (q.v.). See Chronology, 1973, Cohen et al.; bacterial artificial chromosomes (BACs), P1 artificial chromosomes (PACs), Ti plasmid.

Subjects: Genetics and Genomics.


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