protein sequencing

'protein sequencing' can also refer to...

protein sequencing

protein sequencing

protein sequencing

Sequencing covalent modifications of membrane proteins

Spec-seq: determining protein–DNA-binding specificity by sequencing

Assessing protein coding region integrity in cDNA sequencing projects.

Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins

Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus

Top-down analysis of protein samples by de novo sequencing techniques

HUGE: a database for human large proteins identified by Kazusa cDNA sequencing project

HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project

HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project

Sequencing delivers diminishing returns for homology detection: implications for mapping the protein universe

Cloning and Sequencing of the Gene for a Tetrahymena Fimbrin-Like Protein

Identification, N-terminal region sequencing and similarity analysis of differentially expressed proteins in Paracoccidioides brasiliensis

Cloning, sequencing and expression of the flagellin core protein and other genes encoding structural proteins of the Vibrio cholerae flagellum

A Proteomic Approach to Identification of Transmembrane Proteins and Membrane-anchored Proteins of Arabidopsis thaliana by Peptide Sequencing

Cloning and sequencing of SOB3, a human gene coding for a sperm protein homologous to an antimicrobial protein and potentially involved in zona pellucida binding

Cloning, sequencing and functional analysis of Magnaporthe grisea MVP1 gene, a hex-1 homolog encoding a putative ‘woronin body’ protein


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Quick Reference

The process of determining the amino-acid sequence of a protein or its component polypeptides. The technique most commonly used is Edman degradation (devised by Pehr Edman), in which the terminal amino-acid residues are removed sequentially and identified chromatographically. Each step is automated and the whole process can now be performed by a single machine – the sequenator. Large polypeptides must be cleaved into smaller peptides before sequencing.

The results of this chemical sequencing can often be compared with the amino-acid sequence deduced by DNA sequencing. The gene coding for the protein under investigation may be found by screening a DNA library, for example by Western blotting. However, the base sequence of the gene gives only the amino-acid sequence of the nascent protein, i.e. before post-translational modification. The sequence of the functional protein can only be found by chemical analysis.

Subjects: Medicine and Health — Biological Sciences.

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