Overview

protein sequencing


'protein sequencing' can also refer to...

protein sequencing

protein sequencing

protein sequencing

Sequencing covalent modifications of membrane proteins

Spec-seq: determining protein–DNA-binding specificity by sequencing

Assessing protein coding region integrity in cDNA sequencing projects.

Cloning and DNA sequencing of the surface protein antigen I/II (PAa) of Streptococcus cricetus

HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project

HUGE: a database for human large proteins identified in the Kazusa cDNA sequencing project

HUGE: a database for human large proteins identified by Kazusa cDNA sequencing project

Identification, N-terminal region sequencing and similarity analysis of differentially expressed proteins in Paracoccidioides brasiliensis

Sequencing delivers diminishing returns for homology detection: implications for mapping the protein universe

Cloning and Sequencing of the Gene for a Tetrahymena Fimbrin-Like Protein

Top-down analysis of protein samples by de novo sequencing techniques

Discovery of active proteins directly from combinatorial randomized protein libraries without display, purification or sequencing: identification of novel zinc finger proteins

Cloning, sequencing and expression of the flagellin core protein and other genes encoding structural proteins of the Vibrio cholerae flagellum

A Proteomic Approach to Identification of Transmembrane Proteins and Membrane-anchored Proteins of Arabidopsis thaliana by Peptide Sequencing

Miguel García-Sancho, Biology, Computing, and the History of Molecular Sequencing: From Proteins to DNA, 1945–2000

INFOGENE: a database of known gene structures and predicted genes and proteins in sequences of genome sequencing projects

 

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Quick Reference

The process of determining the amino-acid sequence of a protein or its component polypeptides. The technique most commonly used is Edman degradation (devised by Pehr Edman), in which the terminal amino-acid residues are removed sequentially and identified chromatographically. Each step is automated and the whole process can now be performed by a single machine – the sequenator. Large polypeptides must be cleaved into smaller peptides before sequencing.

The results of this chemical sequencing can often be compared with the amino-acid sequence deduced by DNA sequencing. The gene coding for the protein under investigation may be found by screening a DNA library, for example by Western blotting. However, the base sequence of the gene gives only the amino-acid sequence of the nascent protein, i.e. before post-translational modification. The sequence of the functional protein can only be found by chemical analysis.

Subjects: Medicine and Health — Biological Sciences.


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