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A short, synthetic DNA segment containing a restriction site that is coupled to both ends of a blunt-ended restriction fragment. The adaptor is used to join one molecule with blunt ends to a second molecule with cohesive ends. The restriction site of the adaptor is made identical to that of the other molecule so that when cleaved by the same restriction enzyme both DNAs will contain mutually complementary cohesive ends.

Subjects: Genetics and Genomics.

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