Journal Article

Isolation and Characterization of an Enzyme with β-Glucosidase and β-Fucosidase Activities from <i>Dalbergia cochinchinensis</i> Pierre

Chantragan Srisomsap, Jisnuson Svasti, Rudee Surarit, Voraratt Champattanachai, Phannee Sawangareetrakul, Kanokporn Boonpuan, Pantipa Subhasitanont and Daranee Chokchaichamnankit

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 119, issue 3, pages 585-590
Published in print March 1996 | ISSN: 0021-924X
Published online March 1996 | e-ISSN: 1756-2651 | DOI: https://dx.doi.org/10.1093/oxfordjournals.jbchem.a021282
Isolation and Characterization of an Enzyme with β-Glucosidase and β-Fucosidase Activities from Dalbergia cochinchinensis Pierre

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A glycosidase enzyme with both β-glucosidase and β-fucosidase activities has been purified from the seeds of Dalbergia cochinchinensis Pierre (Thai Rosewood) by ammonium sulfate fractionation, preparative isoelectric focusing, and Sephadex G-150 chro-matography. The enzyme has molecular weights of 330,000 in the native state and 66,000 in the denatured state. Hydrolysis of p-NP-β-D-glucoside and p-NP-β-D-fucoside showed pH optimum at pH 5.0 and was inhibited by Rcat-gluconolactone, HgCl2, and p-chloromercuri-benzoate. The Km and fecal values of the purified enzyme were 5.4 mM and 307 s1 for p-NP-β-D-glucoside and 0.54 mM and 151 s−1 for p-NP-β-D-fucoside, so that the latter had by far the higher kcat&Km ratio. p-NP-β-D-galactoside, p-NP-β-D-xyloside, and p-NP-α-L-arabinoside were hydrolyzed more slowly. Hydrolysis of sophorose, laminaribiose, and gentiobiose were also rather slow, and hydrolysis of cellobiose was even slower. No hydrolysis of the cyanogenic glucosides linamarin or prunasin, but some hydrolysis of amygdalin and salicin was found. Further studies are required to identify the natural substrates of the enzyme. However, high yields, ease of purification, and storage stability of the enzyme make it a useful candidate for various applications, such as study of oligosaccharide synthesis by reversal of hydrolysis

Keywords: Dalbergia spp; β-fucosidase; β-glucosidase; glycosidase; rosewood

Journal Article.  0 words. 

Subjects: Biochemistry

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