Journal Article

Effect of Hepatocyte Growth Factor/Scatter Factor on Lipogenesis in Adult Rat Hepatocytes in Primary Culture

Qing Shao, Naokatu Arakaki, Tomokazu Ohnishi, Osamu Nakamura and Yasushi Daikuhara

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 119, issue 5, pages 940-946
Published in print May 1996 | ISSN: 0021-924X
Published online May 1996 | e-ISSN: 1756-2651 | DOI: http://dx.doi.org/10.1093/oxfordjournals.jbchem.a021333
Effect of Hepatocyte Growth Factor/Scatter Factor on Lipogenesis in Adult Rat Hepatocytes in Primary Culture

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Hepatocyte growth factor (HGF)/scatter factor is known to be the most potent mitogen for hepatocytes. In this paper, we report that lipogenesis in primary cultured rat hepatocytes treated with 10 ng/ml of recombinant human HGF (rhHGF) for 24 h was stimulated, as measured by the incorporation of 3H2O into long-chain fatty acids, to more than twice as much as the control. Insulin (0.1 μM) was more effective than rhHGF but rhHGF did not show an additive or synergistic effect when added to insulin. We also showed that treatment with rhHGF increased the activities of glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme, key enzymes which supply NADPH for lipogenesis, and acetyl-CoA carbox-ylase, the rate-limiting enzyme of lipogenesis. The increase in G6PDH and acetyl-CoA carboxylase activities was accompanied by increases in the levels of mRNA for the enzymes. These results suggest that HGF is involved in liver regeneration not only by stimulation of cell proliferation but also by acceleration of differentiation of hepatocytes.

Keywords: acetyl-CoA carboxylase; cell differentiation; hepatocyte; hepatocyte growth factor/scatter factor; lipogenesis

Journal Article.  0 words. 

Subjects: Biochemistry

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