Journal Article

Solution Structure of CINC/Gro Investigated by Heteronuclear NMR

Hiroyuki Hanzawa, Hideyuki Haruyama, Kiyoshi Konishi, Kazuyoshi Watanabe and Susumu Tsurufuji

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 123, issue 1, pages 62-70
Published in print January 1998 | ISSN: 0021-924X
Published online January 1998 | e-ISSN: 1756-2651 | DOI: http://dx.doi.org/10.1093/oxfordjournals.jbchem.a021917
Solution Structure of CINC/Gro Investigated by Heteronuclear NMR

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Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and simulated annealing. The backbone atomic r.m.s. differences for 19 structures about the mean coordinates for residues 7 to 70 are 0.69±0.15 Å for the dimer and 0.56±0.13 Å for the monomer. The N terminal region containing an ELR sequence, an essential motif for chemotactic activity, is disordered in solution, as in other CXC chemokines. The dimer structure consists of a six-stranded anti-parallel β sheet with two C-terminal α helices on the β sheet. The overall dimer structure of CINC/Gro is similar to that of human MGSA, though the backbone r.m.s.d.s between CINC/Gro and the two MGSA structures were high (1.81 and 2.34 Å for the monomer) in spite of the high sequence homology (67%). The major difference resides in the relative position of the C-terminal α helix with respect to the β sheet. This results in a difference of interhelical distances in the dimer: wider (15 Å) in CINC/Gro, as in IL-8, than in MGSA (11.7 and 10 Å).

Keywords: chemokine; dimer; inflammation; NMR

Journal Article.  0 words. 

Subjects: Biochemistry

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