Journal Article

HPC–1/Syntaxin 1A Suppresses Exocytosis of PC12 Cells

Takuya Watanabe, Tomonori Fujiwara, Shinji Komazaki, Kazuhiko Yamaguchi, Osamu Tajima and Kimio Akagawa

in The Journal of Biochemistry

Published on behalf of The Japanese Biochemical Society

Volume 125, issue 4, pages 685-689
Published in print April 1999 | ISSN: 0021-924X
Published online April 1999 | e-ISSN: 1756-2651 | DOI: https://dx.doi.org/10.1093/oxfordjournals.jbchem.a022337
HPC–1/Syntaxin 1A Suppresses Exocytosis of PC12 Cells

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The membrane protein syntaxin (originally named HPC-1) is involved in vesicle trafficking and required for neurotransmitter release at nerve terminals. The presence of syntaxin on target membranes is hypothesized to confer specificity to targeting and fusion via interactions with complementary vesicle-associated proteins. To elucidate the function of syntaxin 1A in exocytosis, HPC-1/syntaxin 1A-reduced PC12h cells (PC12h/Δsyx) that were stably transfected with a plasmid for antisense syntaxin 1A expression were constructed. Depolarizing stimulation of PC12h/Δsyx enhanced dopamine release, compared with PC12h. There was a strong inverse correlation between syntaxin 1A protein expression and enhancement of dopamine release. Reduction of syntaxin 1A had no effect on increase of the cytoplasmic free Ca2+ concentration by depolarized stimulation. Moreover, PC12h/Δsyx clones similarly enhanced of exocytosis by native secretagogues. These results indicate that syntaxin 1A has more than one function in exocytosis.

Keywords: exocytosis; HPC-1; PC12h; SNARE; syntaxin

Journal Article.  0 words. 

Subjects: Biochemistry

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